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Title: A PCR BASED SNP ANALYSIS OF THE CHICKEN TVB RECEPTOR GENE

Author
item Zhang, Huanmin
item Hunt, Henry
item Cheng, Hans
item BACON, LARRY - USDA-ARS-MWA-ADOL

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/6/2003
Publication Date: 7/31/2003
Citation: Zhang, H.M., Hunt, H.D., Cheng, H.H., Bacon, L.D. 2003. A PCR based SNP analysis of the chicken TVB receptor gene [abstract]. Poultry Science. 82(1):49.

Interpretive Summary:

Technical Abstract: In the early 1970s, multiple alleles of the chicken TVB receptor gene were defined in domestic chickens. A specific functional receptor is required for successful cellular infection by a particular subgroup of avian leukosis virus (ALV). The TVB*S1 allele encodes a receptor for exogenous ALV subgroups B and D, and endogenous ALV subgroup E. TVB*S3 encodes a receptor for the subgroups B and D but not for subgroup A. In contrast, the TVB*R allele does not encode a functional receptor for any ALV subgroup. Recent reports indicate that two single nucleotide polymorphisms (SNP) are responsible for the allelic differences. To expedite the genotyping of the TVB locus, two sets of PCR primers were developed to amplify chicken genomic DNA encompassing the SNPs. One of the two sets employed a mutagenic primer to introduce an Xba I restriction enzyme site during PCR amplification to facilitate direct endonuclease analysis of the first SNP. Following digestions of the two amplicons with restriction endonucleases Nal III and Xba I and agarose gel electrophoresis, specific TVB genotypes, defined by the reported SNPs, were determined by examining the banding patterns of one or both amplicons. Results to confirm this rapid genotyping system in experimental chicken crosses will be presented. If the phenotypes agree with the genotypes, then this PCR-based system may be useful for screening TVB alleles susceptible or resistant to specific ALV subgroups in other chicken lines and populations. Key Words: Chicken, Avian Leukosis Virus, TVB gene, Genotypes.