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Title: SEQUENCING OF EXPRESSED SEQUENCE TAGS (ESTS) UNIQUE TO THE BOS TAURUS GENE INDEX

Author
item Connor, Erin
item Sonstegard, Tad
item Van Tassell, Curtis - Curt

Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: 12/27/2002
Publication Date: 12/27/2002
Citation: Connor, E.E., Sonstegard, T.S., Van Tassell, C.P. 2002. Sequencing of expressed sequence tags (ests) unique to the bos taurus gene index. Germplasm Release. Accession Numbers CA923315-CA923384.

Interpretive Summary: Germplasm release. No interpretive summary required.

Technical Abstract: In a previous study, a normalized cDNA library was developed from pooled mammary gland tissues and sequenced. Expressed sequence tags were assembled into tentative consensus (TC) sequences and compared by BLAST to the gene indices of other species and the human genome assembly. Those TCs identified as unique to the Bos taurus gene index and with no assignment to the Eukaryotic Gene Orthologs (EGO) database were selected for additional sequencing. Clones (n = 69) comprising the 3' end of each TC were sequenced and incorporated into new TC assemblies. These data will be used to characterize previously unidentified transcripts and identify those which may represent genes unique to ruminants.