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United States Department of Agriculture

Agricultural Research Service

Title: Genomic Fingerprinting of Salmonella Recovered from Swine Carcass and Fecalsamples at a Slaughterhouse

item Wonderling, Laura
item Wallace, Frederick
item Call, Jeffrey
item Tamplin, Mark
item Feder, Ingrid
item Luchansky, John
item Pearce, Rachel - DUBLIN IRELAND
item Palumbo, Samuel - NAPERVILLE IL

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 1, 2002
Publication Date: June 30, 2002
Citation: Wonderling, L.D., Wallace, F.M., Call, J.E., Tamplin, M.L., Feder, I.E., Luchansky, J.B., Pearce, R., Oser, A., Palumbo, S. 2002. Genomic fingerprinting of salmonella recovered from swine carcass and fecalsamples at a slaughterhouse. International Associate for Food Protection. Abstract #12, p.87.

Technical Abstract: Previously, 100 swine carcasses were swabbed and 60 corresponding fecal samples were collected on 10 days over a 30-day period. From carcass (73%) and fecal (33%) samples that were positive for Salmonella, a total of 582 isolates were characterized by PFGE. There were 13 unique XbaI restriction profiles, with the majority (71%) of the isolates having profile types "F" (36%) or "B" (35%). While both the "F" (S. Typhimurium DT104-like PFGE profile) and "B" profiles were found on at least 8 of 10 sampling days, profile "I" was found on 3 days, profiles "A" and "D" on 2 days, and the remaining 8 profiles on only 1 of the 10 sampling days. A single isolate obtained from a carcass 18 hours post-chill during the survey period also displayed profile type "F". Examining multiple isolates from a given carcass revealed a single profile in 40 of 57 (70%) samples tested. Similarly, multiple isolates from a given fecal sample displayed a asingle profile in 16 of 19 (82%) samples tested. In general, analyses of the 15 paired carcass-fecal samples revealed that clonal types found in feces were typically found on the carcass and vice-versa, suggesting that fecal isolates from a given animal contaminated the carcass. These data suggest that although each group of animals can introduce new clonal types into the slaughterhouse, the majority of Salmonella associated with a given animal exhibit low genomic diversity.

Last Modified: 4/18/2015
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