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Title: GENERATION OF A SUBGROUP J AVIAN LEUKOSIS VIRUS INFECTIOUS CLONE EXPRESSING GREEN FLUORESCENCE PROTEIN AND ITS POTENTIAL USES

Author
item LUPIANI, BLANCA - MICHIGAN STATE UNIVERSITY
item Hunt, Henry
item CONKLIN, KATHLEEN - UNIVERSITY OF MINNESOTA
item REED, WILLIE - MICHIGAN STATE UNIVERSITY
item Fadly, Aly

Submitted to: North Central Avian Disease Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/2/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: During the last decade, subgroup J avian leukosis virus (ALV-J) has been associated with serious mortality and other production problems in meat-type chickens world wide. ALV-J isolates have been shown to be very variable both at the genetic and antigenic level. In this report, we describe the generation of an infectious molecular clone of strain ADOL-R5-4 of ALV-J (pR5-4). This molecular clone is in the form of proviral DNA and is infectious upon transfection into chicken cells. pR5-4 has also been modified to include a splice acceptor site after the viral env gene as well as a unique restriction site that allows the cloning of foreign genes into the virus (pR5-4 SA). In order to test the stability of this viral clone we have cloned a soluble modified form of the green fluorescence protein (smGFP) into this unique site in pR5-4 SA. The resultant plasmid, pR5-4 SA smGFP, has been shown to be very stable even after 3 month of passaging in tissue culture. This new molecular clone is an essential tool in our attempts to study cell tropism of ALV-J in both meat- and egg-type chickens.