|Palukaitis, Peter - CORNELL UNIVERSITY|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2001
Publication Date: December 15, 2001
Citation: Lee, L., Gray, S.M., Palukaitis, P. 2001. Systemic movement of 17k and cp mutants of plrv in nicotiana species. Phytopathology. 91:S54. Technical Abstract: The Potato leafroll virus (PLRV) coat protein (CP) and 17kDa movement protein (17K) are translated from nested ORFs and both proteins influence systemic infection of Nicotiana species. The systemic infection of several PLRV mutants, one that does not translate the 17K and five alanine-scanning CP mutants were followed in N. benthamiana using immuno-tissue blot analysis. The 17K mutant followed a path similar to wild-type virus (WT), accumulating to detectable levels in external and internal phloem, but the mutant infected fewer cells. Analysis of the systemically infected leaves by ELISA also confirmed that the 17K mutant accumulated less virus than WT. The CP mutants were able to infect systemically N. clevelandii, but three mutants containing substitutions in the interior of the CP sequence did not accumulate to WT levels and virions could not be purified from tissue infected systemically. The 17K and CP of PLRV play important roles in systemic movement, the 17K in the infection efficiency of phloem and the C in the assembly and/or stability of the virus particles.