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United States Department of Agriculture

Agricultural Research Service

Title: Genomic Expression Profiling in Soybean Plants

Authors
item Khan, Rana - UNIVERSITY OF MARYLAND
item Alkharouf, Nadim - GEORGE MASON UNIVERSITY
item Beard, Hunter
item Macdonald, Margaret
item Knap, Halina - CLEMSON UNIVERSITY
item Matthews, Benjamin

Submitted to: Mid Atlantic Plant and Molecular Biology Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 30, 2001
Publication Date: N/A

Technical Abstract: The interaction between the soybean plant and soybean cyst nematode (SCN) may result in resistance or disease depending upon the variety of soybean and the race of SCN invading the plant. The economic loss caused by SCN in the U.S. is significantly greater than any other known pathogen of soybean and necessitates an understanding of the mechanisms underlying the basis of resistance to SCN, in order to develop more resistant varieties. Identification of plant host genes involved in defense responses is an important step towards elucidation of disease resistance mechanisms in plants. We have used microarray technology to examine the expression profile of approximately 1200 soybean root genes in a resistant and susceptible soybean cultivar two days after nematode invasion. The array was generated by spotting PCR amplified inserts from cDNA clones derived from the roots of an SCN-infected resistant soybean variety and were hybridized with fluorescently labeled probes prepared from total RNA isolated from SCN-infected roots of resistant or susceptible soybean varieties. In each case the uninfected susceptible variety was used as a control. Data analysis reveals that approximately 10% of the 1200 genes are statistically induced in both cultivars. Among the genes that are uniquely induced in either the resistant or the susceptible cultivars, a large number are proteins of unknown function. The others appear to be involved in general defense responses and fall into the categories of cell wall maintenance or development, synthesis of secondary metabolites, stress induced, and metabolic pathways.

Last Modified: 4/20/2014
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