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United States Department of Agriculture

Agricultural Research Service

Title: Advances in DNA Markers

Authors
item Cregan, Perry
item Quigley, Charles
item Song, Q - VISITING SCIENTIST CHINA
item Zhu, Y - VISITING SCIENTIST CHINA

Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: August 14, 2000
Publication Date: N/A

Technical Abstract: The Minsoy x Noir genetic linkage map released in 1998 contained 36 intervals of 20 cM or greater in which no SSR marker had been positioned. Since that time at least one new SSR locus has been mapped to 30 of the 36 intervals using a combination of targeted and random marker development. The number of mapped soybean SSR markers now exceeds 1000. We are proceeding with the development of approaches for the discovery and mapping of single nucleotide polymorphisms (SNPs). A major emphasis is being placed upon the discovery of SNPs in the UniGenes that are being assembled and annotated as part of the USB- funded Soybean EST Project (R.C. Shoemaker) and the NSF-funded Soybean Functional Genomics Project (L. Vodkin et al.). SNPs in the UniGenes will be mapped using the single base extension (SBE) approach with analysis of SBE products using denaturing high performance liquid chromatography or the Perkin-Elmer 377 and 3100 DNA analyzers. This effort will create a large number of new SNP markers and will permit the positioning of transcripts on the existing recombination map of the soybean genome.

Last Modified: 4/23/2014
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