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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sugarbeet and Potato Research » Research » Publications at this Location » Publication #116933
Title: MOLECULAR GENETIC TAGGING OF RESISTANCE IN SUGARBEET TO ROOT KNOT NEMATODE (MELOIDOGYNE SPECIES).

Author
item Weiland, John
item Yu, Ming

Submitted to: American Society of Sugarbeet Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 11/13/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Incorporation into sugarbeet (Beta vulgaris L.) of resistance to root knot nematode has been an ongoing project at the USDA-ARS laboratory in Salinas, CA. Resistance discovered in Beta maritima appears to be simply inherited and is effective against multiple species and races of nematode belonging to the genus Meloidogyne. Sugarbeet population accession 1568 segregating for a resistance to root knot nematode was used in an effort to develop molecular genetic markers tagging this resistance. Preparations of DNA were made from leaves of plants that exhibited susceptibility or resistance to nematode after inoculation in a greenhouse. Pooled DNA of segregants was used to identify markers associated with the resistance using the random amplified polymorphic DNA technique. At least 5 DNA markers were obtained that co-segregated with nematode resistance. One marker was coupled in repulsion to the resistance. The use of this marker in the incorporation of nematode resistance into elite sugarbeet breeding germplasm is discussed.