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ARS Home » Research » Publications at this Location » Publication #113539
Title: IDENTIFICATION OF GENES AND PATHWAYS FOR RESISTANCE TO MAREK'S DISEASE THROUGH DNA MICROARRAYS

Author
item Liu, Hsiao Ching
item Cheng, Hans
item SOFER, L - U OF DELAWARE, NEWARK DE
item BURNSIDE, JOAN - U OF DELAWARE, NEWARK DE

Submitted to: World Poultry Congress Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/20/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: DNA microarrays contain thousands of cDNAs gridded at high density on solid supports. By comparing two or more samples, a large amount of quantitative information is produced which leads to insights on the potential relationships between genes and traits. DNA microarrays have been developed using a T lymphocyte-enriched cDNA library; see the U of Delaware eweb site at: udgenome.ags.udel.edu/chickest/array.htm for more information Examination of 1,200 ESTs indicated that ~155 were differentially expressed between uninfected or MDV-infected Line 6 (MD resistant) and Line 7 (MD susceptible) peripheral blood lymphocytes (PBLs). Grouping these genes by biological pathways and similar kinetics shows that the results are consistent with previous literature as well as providing insights on the specific molecular mechanisms involved in MD resistance. Presently, we are mapping these genes to see if they lie in one of our 14 QTL that confer resistance to MD. If so, then these genes would be positional candidate genes and good targets for further analysis. Thus, DNA microarrays combined with genetic mapping provide a powerful combination for identifying MD resistance genes and the biological pathways involved.