Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 6/23/1999
Publication Date: 2/1/2000
Citation: GUSTAFSON, J.P., YANO, M. GENETIC MAPPING OF HYPERVARIABLE MINISATELLITE SEQUENCES IN RICE (ORYZA SATIVA L.). JOURNAL OF THEORETICAL AND APPLIED GENETICS. 2000. V. 100(3-4). P. 447-453. Interpretive Summary: A class of DNA sequences has been long known in humans that are called "DNA fingerprinting" sequences. These sequences have been used for years in human-related cases involving forensics, paternity, and disease mapping. By using pieces of the human sequences, we isolated and characterized a series of similar "DNA fingerprinting" sequences from cultivated rice. This report describes the research designed to ascertain the distribution of these sequences throughout all of the rice genome. If the sequences are to be used for DNA fingerprinting in rice, then it is important to know if they are scattered throughout all of the rice chromosomes. The results indicated that the sequences are located on every rice chromosome. The impact of this research is that rice breeders and geneticists who are fingerprinting rice cultivars in the United States and rice germplasm from around the world will be able to identify genetic differences between morphologically similar cultivars.
Technical Abstract: Minisatellites have been utilized in animal linkage studies for several years. Minisatellites, or DNA fingerprinting sequences, have not generally been used as markers for plant genome mapping. In animal genome mapping, they have resulted in some success because they are evenly dispersed in some species, but are often clustered near telomeric regions of human chromosomes. The purpose of the present study was to generate DNA fingerprints, utilizing several minisatellites containing different core sequences and numbers of repeat units, followed by assessing their potential for use as genetic markers when mapped to a rice recombinant inbred line (RIL) population. Sites of segregating minisatellite loci were mapped onto 11 of the 12 rice RIL linkage maps. The implications for the use of rice minisatellite core sequences as genetic markers on linkage maps in rice are discussed.