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ARS Home » Research » Publications at this Location » Publication #99785
Title: AFLP AND RAPD MARKERS LINKED TO PHYTOPHTHORA FRAGARIAE RESISTANCE GENES IN STRAWBERRY

Author
item HAYMES, KENNETH - CONTRACT EMPLOYEE
item Hokanson, Stan
item VAN DE WEG, ERIC - CPRO-DLO
item Maas, John

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: 4/8/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The commercial strawberry is susceptible to red stele root rot caused by the fungus Phytophthora fragariae var. fragariae. Red stele resistance in strawberries and virulence of P. fragariae behave according to the gene-for-gene system. The goal of this international research project with CPRO-DLO, Wageningen, The Netherlands, is to develop highly specific molecular markers for various Rpf resistance genes (Rpf1, Rpf2, Rpf3, and Rpf6) that confer resistance against P. fragariae. Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphic (AFLP) markers putatively linked to P. fragariae resistance genes. The bulked DNAs represent subsets of three F1 populations that segregated monogenically for either resistance or susceptibility to P. fragariae. The map of these Rpf loci was generated using JoinMap. The RAPD markers linked to the resistance genes are being converted into sequence characterized amplified region (SCAR) markers while the AFLP markers will be used as probes for the detection of P. fragariae. Marker-facilitated selection of these resistance genes would allow an efficient means in the screening and selection of plant material containing these genes and help in the pyramiding for resistance to P. fragariae. Genetic improvement of the strawberry by the ability to pyramid resistance genes contributes directly to strawberry breeding programs and to the productivity of the strawberry industry worldwide.