Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/1998
Publication Date: N/A
Technical Abstract: The early events in the infection process of wheat spikes by Fusarium graminearum Schw. were examined. Glumes from spray inoculated spikes of Sumai (resistant) and Wheaton (susceptible) were sampled at 6, 12, 24, 36, 48 and 76 hours after inoculation and examined using scanning electron microscopy. Conidia germination occurred between 6-12 hours. Between 24-48 hours some subcuticular hyphal development was evident and other hyphae were found to penetrate stomata. By 76 hours after inoculation, conidiophores developed on infected glumes. No major differences in the timing of these events were found between the two cultivars. Additionally, RNA was extracted from whole wheat spikes collected from the two cultivars at the same timepoints. RNA blot analysis was used to determine the expression pattern of several defense genes in the spikes during Fusarium infection.