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ARS Home » Research » Publications at this Location » Publication #98366
Title: DETECTION OF A SMALL ANIONIC ANTIMICROBIAL PEPTIDE IN BOVINE BRONCHIAL ALVEOLAR LAVAGE FLUID AND LUNG

Author
item FALES-WILLIAMS, A - ISU, VET. MED., AMES, IA
item Brogden, Kim
item HUFFMAN, E - ISU, VET. MED., AMES, IA.
item ACKERMANN, M - ISU, VET. MED., AMES, IA.

Submitted to: American Society of Microbiologists Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/30/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Anionic peptide (AP) in both ovine and human BAL fluid and lung tissues have broad-spectrum bactericidal activity. The presence of AP in cattle has not been established, nor has the extent of cellular production of AP been determined in normal and pneumonic lungs of sheep and cattle. In this study, cattle were inoculated with either live Pseudomonas aeruginosa, purified LPS extract, or pyrogen-free saline by bronchoscopy then euthanasized 4-6 hours after inoculation. Monoclonal antibody to AP (AP-mAb) was used to detect AP-containing proteins in western blots of lung homogenates. AP-mAb reacted with four bands: 28-30 kDa (strongest signal), 31 kDa, variable band in calves at 45 kDa and at 35-38 kDa in the cow, and a 64 kDa band present in adult only. Band sizes were consistent with those of ovine AP and are immunoreactive to AP-mAb; thus the anionic peptide of ovine BAL fluid is also native to the bovine. The distribution of AP immunoreactivity (AP-IR) was also determined by immunocytochemistry in lungs of sheep and cattle that received pyrogen-free saline, Pasteurella haemolytica, purified LPS, or LPS-associated peptide. AP-IR was present in both sheep and cattle lung sections, with positive staining of airway epithelium of all airways, submucosal glands, and smooth muscle cells. While apical cytoplasm of airway epithelium was stained, nuclear staining of epithelial cells, smooth muscle, and submucosal glands was more consistent. AP-IR was highest in animals that received saline or LPS- associated protein rather than those inoculated with LPS or live bacteria.