Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/9/1998
Publication Date: N/A
Technical Abstract: The soybean cyst nematode (SCN), Heterodera glycines Ichinohe is a major pest of the soybean worldwide and is responsible for millions of dollars worth of damage in the USA. There are numerous races of SCN and control of damage can be difficult. A cDNA library was constructed from mRNA isolated from soybean leaves and roots of the resistant variety "Peking" two days after invasion by SCN, race 3. An array of 384 clones were isolated and one-passed sequenced from one end to provide expressed sequence tags (ESTs). Four genes were most abundant in the array. Three encoded genes with moderate identity to Beta-galactosidase (72%), soybean hydroxyproline rich (HPR) glycoprotein (76%), a zinc finger protein (68%), while the fourth was not represented in Gen Bank. Other clones of lower abundance had moderate homology at the amino acid level to genes encoding enzymes involved in the phenylpropanoid pathway leading to glyceollin and lignin synthesis, numerous protein kinases, peroxidases, and some "house-keeping" genes. This array is being used to identify relationships, functions and identities of soybean genes important in defense against nematodes.This information is useful to scientists studying genes involved in pest resistance.