Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/1998
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Expression of the Beta (B) locus in tomato increases fruit beta-carotene content at the expense of lycopene and results in increased orange fruit pigmentation. The degree to which B is expressed is influenced by the beta-modifier (Mo-B) gene which segregates independently of B. Amplified fragment length polymorphism (AFLP) analysis was performed using a pair of near isogenic lines (NILs) designed to be isogenic at the B locus. NILs were derived from an initial cross of Lycopersicon esculentum cv. Rutgers x L. hirsutum. Using 64 AFLP primer pairs, polymorphic fragments were identified which distinguished the NILs. A 100 bp AFLP amplification product was identified which was unique to the red-fruited NIL. Analysis of an F-2 population derived from a cross of the NILs confirmed that the 100 bp product cosegregated with MoB and was tightly linked to this locus. The marker exhibited a recombination frequency of 1.7%. This MoB-linked AFLP marker was cloned and sequenced. The sequences were used to design 24-mer oligonucleotide primers for respective clones and develop sequence characterized amplified region (SCAR) markers for use in marker-aided selection, high-resolution linkage analysis, and map-based cloning.