Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/6/1998
Publication Date: N/A
Interpretive Summary: A portion of the gene encoding Androgen Receptor was amplified and sequenced in the pig. A genetic marker was developed and this marker was typed across the USDA, ARS, U.S. Meat Animal Research Center (MARC) Swine Reference Population, as well as a somatic cell hybrid panel. Analyses of the data determined that androgen receptor mapped to the X chromosome of the pig. Assignment of this gene provides another reference point for a comparison between the order of genes on the porcine and human X chromosomes.
Technical Abstract: Primers were developed from bovine and human sequence to amplify intron seven of the androgen receptor gene in swine. Pig specific primers were developed which flanked a microsatellite within the intron. The marker was genotyped across the USDA, ARS, U.S. Meat Animal Research Center (MARC) Swine Reference Population and linkage analyses were conducted. Four alleles were identified in the MARC population as well as in samples from 55 unrelated swine representing five breeds. In addition, androgen receptor was also typed across a porcine somatic cell hybrid panel. Androgen receptor mapped to position 60 cM on the MARC swine genetic map for chromosome X and to the q13 band of the X chromosome based on the somatic cell panel. Assignment of this gene provides another reference point for the porcine-human comparative map of the X chromosome.