Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: 7/15/1998
Publication Date: N/A
Technical Abstract: The soybean cyst nematode (SCN), Heterodera glycines Ichinoe is a devastating pest of the soybean, Glycine max L. Merr, causing chlorosis, root necrosis, and suppressing shoot growth. We used differential display to identify genes expressed in a resistant soybean cultivar (Peking) compared to susceptible (Kent) and uninoculated plants. Gene expression was monitored 6, 12, 24, 48, 96 and 144 hours after inoculation with SCN. We identified and cloned over 50 unique DNA fragments by differential display, some which correspond to genes specifically induced in leaves or roots of resistant inoculated plants. The DNA sequences were compared against nucleotide and protein databases to identify putative gene function. We identified genes induced at an early stage of the infection process, such as transcription factors, nucleotide binding proteins, protein kinases and leucine-rich repeats. These genes are induced during nematode penetration and migration through the root. At a later stage, genes involved in general defense response were induced, coinciding with degeneration of the nematode feeding site. cDNA arrays are being tested to determine the response of other soybean genes to SCN invasion.