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ARS Home » Research » Publications at this Location » Publication #95504
Title: STUDIES ON CHARACTERISTICS AND DISTRIBUTION OF RED LASODA VIRUS (RLSV) IN NORTH AMERICA

Author
item Goth, Robert
item ELLIS, PETER - PHYTO DIAGNOSTICS CO.
item DE VILLIERS, GERDA - PHYTO DIAGNOSTICS CO.
item Goins, Ernest
item WRIGHT, N. - PHYTO DIAGNOSTICS CO.

Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/31/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: In 1992 and 1993 a carlavirus (code name RLSV) with serological and biological characteristics different from potato virus M (PVM) and potato virus S (PVS) was detected in the potato cv Red LaSoda. A monoclonal antibody that could be used to serologically detect this virus was developed. Serological tests using this antibody detected nine naturally infected potatoes and two from the US Varietal collection at Presque Isle, ME. Three cultivars(High Plains, Platte and Red LaSoda) tested positive for RLSV in 1997 and 1998. New systemic bioassay hosts for RLSV are Nicotiana benthimiana, N. megalosiphon and N. occidentalis. Currently an ELISA method using the 4E12 Mab is the standardized RLSV test for importation of nuclear seed potato stocks into Canada. This information will be useful in developing protocols to detect RSLV and thereby enable potato seed producers to meet nuclear seed exportation standards.

Technical Abstract: In 1992 a carlavirus (code name RLSV) with serological and biological characteristics different from potato virus M (PVM) and potato virus S (PVS) was detected in the potato cv. Red LaSoda by the Scottish Agricultural Science Agency. In 1993 a long filamentous rod-shaped virus similar to, but serologically distinct from PVS and PVM, was also revealed in a clone of the potato cv Red La Soda grown in California. The virus was isolated, purified and two highly specific monoclonal antibodies 4E12 and 6H11 were developed. When accessions from the Vancouver Collection of Virus-Free potatoes from 1994-1998 were assayed for RLSV by TAS-ELISA with Mab 4E12 seven (two in 1994, two in 1995 and three in 1996) were infected. None was infected in 1997 and 1998. When the 137 accessions in the US National Potato Varietal Collection maintained at Presque Isle, ME were assayed for RLSV with TAS ELISA with Mab 4E12 in 1997 and 1998, three cultivars(High Plains, Platte and Red LaSoda) tested positive. New systemic hosts for RLSV are Nicotiana bentimiana, N. megalosiphon and N. occidentalis. TAS ELISA using the 4E12 Mab is the standardized RLSV test for nuclear seed potato stocks in Canada.