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ARS Home » Research » Publications at this Location » Publication #95319

Title: DEVELOPMENT OF GENOME INTEGRATED PHYSICAL MAP: POSITIONAL CLONING OF THE CYST NEMATODE RESISTANCE LOCI IN SOYBEAN.

Author
item MEKSEM, K
item Cregan, Perry
item LIGHTFOOT, D
item ARELLI, P
item ZHANG, H

Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: 8/31/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Inheritance of field resistance to soybean cyst nematode (SCN) race 3 in soybean cultivar Forrest is conditioned by two loci: rhg1 on molecular linkage group (MLG) G and Rhg4 of MLG A2. Bulked segregant analysis and AFLP=s were used to select molecular markers closely linked to rhg1 and Rhg4. Two AFLP markers place rhg1 within a 1 cM interval and three AFLP markers place Rhg4 within 0.5 cM. A new type of vector will accelerate the cloning of Rhg4, rhg1 and other agronomically important genes. This vector is able to accept large DNA fragments and to transform plants directly. We have constructed a Bacterial Artificial Chromosome (BAC) library of cultivar Forrest. This library will provide clones for physical mapping of the genome and for chromosome walking and landing. Five BAC clones were identified in the 1 cM interval carrying rhg1 that were shown to overlap by fingerprinting. One clone, A109-4 contains the two AFLP markers flanking either side of rhg1 in Forrest. Sequencing of subclones of A109-4 has identified candidate resistance genes. Transformation with candidate genes from the A109-4 clones is in progress.