Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: 8/28/1998
Publication Date: N/A
Technical Abstract: There is now a growing interest in single nucleotide polymorphisms (SNPs), primarily as a result of the efforts of the Human Genome Project. SNPs, which are point mutations, have been shown to be the most abundant type of DNA polymorphism in a number of species. Their average frequency in humans is 2 to 3 SNPs/kbp. SNPs occur throughout the genome in both coding and non-coding regions, the majority being found in non-coding regions. As a result of their abundance, SNPs have become an important source of molecular markers that contain a wealth of genetic information. One attractive feature of SNPs is the possibility of detection using non-gel based systems. As a result of technologies developing from the Human Genome Project, high-throughput, automated systems have already been developed that allow the parallel assay of SNPs at very high rates. We determined the frequency of SNPs with 12 soybean genes (exons, introns, 5' UTR and 3' UTR) chosen from GenBank and in flanking regions of SSR-containing sequences. The overall level of SNPs in 18 soybean genotypes in a of more than 18,000 basepairs of DNA analyzed was 1 SNP per 295 bases. This frequency is substantially higher than in human and is sufficiently high to provide the basis for the generation of genetic maps based upon SNPs.