Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 8/28/1998
Publication Date: N/A
Technical Abstract: In wheat, microsatellite markers show a higher level of polymorphism than RFLP or RAPD markers. Our objectives were to determine the abundance of trinucleotide microsatellites and the rate at which they can be developed into informative markers. A genomic library from Chinese Spring' (CS) was screened with 5 trinucleotide probes and about 1.1% of the clones contained microsatellites. Among the clones sequenced, 18, 16, 6, 3, and 0 contained ATT, CTT, GTT, ATC, and ACT of 8 or more perfect trinucleotide repeats, respectively. A total of 31 PCR primers sets could be designed and used in amplification of genomic DNA of PI 428203 (AA genome), PI 560527 (BB genome), Triticum tauschii (DD genome), CS, M6 and Opata'. Fourteen of these primer sets amplified single products, 3 amplified 2 products, 14 amplified multiple or smeared products. Eight markers specific to A, B, or D genomes were found, among them, 6 were polymorphic among CS, M6 and Opata. Preliminary results indicate that high quality trinucleotide microsatellite markers can be developed in wheat.