Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/27/1998
Publication Date: N/A
Technical Abstract: To compare the molecular difference and relatedness between Fusarium solani f. sp. glycines that causes soybean sudden death syndrome and other F. solani isolates, the mitochondrial small subunit rDNA (Mt SSU) was PCR-amplified and sequenced from 35 isolates including 14 F. solani f. sp. glycines and 21 F. solani from various plant hosts. All F. solani f. sp. glycines isolates had identical sequences. Two major lineages were found in F. solani isolates in view of the nucleotide similarity, and presence and absence of insertions. Genetic distance values ranged from 0.0178 to 0.0779 between F. solani f. sp. glycines and F. solani isolates. Maximum parsimony analysis, UPGMA, and neighbor-joining analysis gave identical tree topology. Based on the sequence data, a pair of primers, Fsg1 and Fsg2, were designed. Of 93 isolates tested, this primer specifically amplified DNA from all 33 F. solani f. sp. glycines isolates, but not from 45 F. solani and 15 other soybean pathogen isolates.