|Abbott Dr, Thomas|
Submitted to: Industrial Crops and Products
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/15/1999
Publication Date: N/A
Citation: N/A Interpretive Summary: Jojoba is a new crop, native to the U.S. and being grown commercially. The oil from jojoba has a good market, but the rest of the seed is not generally used. We previously developed an industrial scale process for extracting materials from jojoba which make animals feel full and may be useful for controling weight in pets. However, the extract was only 42% pure. In this work we developed a practical process to refine those materials to greater than 90% purity so that the effects of the pure materials can be tested.
Technical Abstract: Simmondsin and several related compounds are present in jojoba meal at about 13% concentration. Industrial scale processes have been developed to extract a simmondsin concentrate that contains 42% simmondsin and analogues. This research was undertaken to develop a practical process to further purify the active constituents. Acetone, acetonitrile, chloroform, isopropanol, methanol, and water, alone and in various combinations and at various solvent:solids ratios were tested. A solvent extraction with absolute ethanol followed by 80/20 ethanol/water increases the concentration of simmondsins from 42 to 71% in the ethanol extract, recovers the remaining simmondsins in the second extract and leaves a sugar and protein residue low in residual simmondsins. Subsequent preparative chromatography gave fractions greater than 95% purity. From the preparative HPLC, 77% of the recovered fractions are pharmaceutical grade and 20% are acceptable for further refinement or recycling into the feed solution.