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Title: DETECTION OF SMALL, ANIONIC ANTIMICROBIAL PEPTIDES IN BRONCHOALVEOLAR LAVAGE FLUID AND RESPIRATORY EPITHELIUM OF PATIENTS WITH AND WITHOUT CYSTIC FIBROSIS.

Author
item Brogden, Kim
item ACKERMANN, M
item MCCRAY, P
item HUTTNER, K

Submitted to: Annual North American Cystic Fibrosis Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/13/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2 ibodies were prepared to H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant. Both antibodies were used to assess the concentration of AP-like molecules in native human bronchoalveolar lavage fluids (47 patients) and in pulmonary tissues (6 patients). Concentrations of AP-like molecules detected with antibody 1G9-1C2 were significantly lower in 13 patients with cystic fibrosis (0.78 mM +/- 0.46 mM SD; P = 0.01) than in 34 patients without cystic fibrosis (1.30 mM +/- 0.66 mM SD). Concentrations of AP-like molecules detected with antibody PAB96-1 were similar and also significantly different between groups (P<0.001). By immunohistochemistry of pulmonary tissues, antibody 1G9-1C2 stained accumulated antigen in the apical cytoplasm of the bronchial and bronchiolar epithelium, in the cytoplasm of pulmonary endothelial cells and on occasional alveolar macrophages. Patients with cystic fibrosis had peribronchial and peribronchiolar lymphocytic infiltrates containing some stained lymphocytes. Neutrophils and suppurative exudate did not stain. Cytoplasmic staining of alveolar type I epithelium was present in 3/3 tissue sections from patients without cystic fibrosis but not in 3/3 tissue sections from patients with cystic fibrosis. These observations form the foundation for further work to isolate and characterize AP-like molecules in the human airway and to assess their role in innate pulmonary defense. Supported by the Cystic Fibrosis Foundation (BROGDE97Z0)