Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/9/1998
Publication Date: N/A
Technical Abstract: Linkage maps are essential for the chromosomal positioning of quantitative trait loci (QTL) in crop plants, and to identify genetic tags for marker-assisted enhancement programs. The amplified fragment length polymorphism (AFLP)technique has been used to quickly assign markers to new mapping populations. The objectives of this study were to: 1) determine which AFLP primer pairs yield the most genetic information among oat mapping parents, and 2) construct a low-density oat linkage map with AFLP, isozyme, and RAPD markers. DNA was extracted from a 'Ogle'/'TAM O-301' recombinant inbred population, and surveyed for AFLP using the procedures provided by Gibco BRL, Life Technologies, Inc. Protocols for isozyme and random amplified polymorphic DNA (RAPD) are referenced. Some AFLP primers were better than others in producing new markers. The newly found markers will be combined with restriction fragment length polymorphisms (RFLPs) which are being assigned to the same population in another laboratory. The new map will be used to further QTL studies in oat, and this will set the stage for future marker-assisted gene transfer studies.