Skip to main content
ARS Home » Research » Publications at this Location » Publication #93048
Title: CORRESPONDENCE BETWEEN PHYLOGENETIC RELATIONSHIPS AND BIOLOGICAL CHARACTERISTICS OF PRUNUS NECROTIC RINGSPOT VIRUS

Author
item Hammond, Rosemarie
item Crosslin, James
item PASINI, RITA - UNIVERSITY OF MARYLAND
item HOWELL, WILLIAM - WASHINGTON STATE UNIV
item MINK, GAYLORD - WASHINGTON STATE UNIV

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 6/29/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Prunus necrotic ringspot ilarvirus (PNRSV), a serious pathogen of many woody species, exists as a number of biologically distinct isolates differing in host specificity, serology, and pathology. Nucleotide sequence alignment and phylogenetic analysis of RT-PCR products obtained from RNA 3 of several isolates representative of differences in pathotype, serotype, and woody host source reveal predictable as well as unpredictable relationships between the isolates. Based upon these analyses, RT-PCR assays have been developed using oligonucleotide primers that can discriminate between isolates displaying different symptoms and serotypes. The technique has been used for detection and differentiation of the isolates and to examine the biological diversity within the PNRSV virus group. The technique also has great potential for use in control of PNRSV-induced diseases in its ability to identify potential cross-protecting strains.