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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #92938


item CLARKE, R.
item Corsini, Dennis
item BERGER, P.

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Viruses are one of the major problems facing potato growers, particularly seed growers. Inspections of seed fields and special laboratory tests are used to detect and eliminate infected plants so that the crop remains disease free. When new strains of the virus appear, the existing test procedures sometimes fail. This was the case with a new strain of virus M, one of the less common potato viruses. We were able to identify and describe this new strain and develop a recommended test procedure to monitor it in the future.

Technical Abstract: In 1994, potato samples for certification from Idaho seed fields reacted in enzyme-linked immunosorbent assay (ELISA)tests to a polyclonal potato carlavirus M (PVM) antiserum. Sample affinity to the antiserum was lower than control samples. Furthermore, ELISA-positive samples were obtained from both symptomatic as well as asymptomatic plants. A complementary DNA library was prepared using both reverse transcription- polymerase chain reaction and primers based on published PVM sequences, or oligo d(T) primed reverse transcribed sequences. The nucleotide sequence was determined for the 3'-terminus of the genome. Putative coat protein amino acid sequence was compared to published PVM and potato virus S coat protein sequences. While this new isolate is likely a strain of PVM, it is significantly different from known PVM coat protein sequences in the amino terminus region. These differences may explain the poor reactivity to other PVM antisera and suggest that it is a new strain of PVM, which we have designated PVM-ID.