Submitted to: American Oat Workers Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 7/13/1998
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: A population from a four-way cross was used to develop barley yellow dwarf virus (BYDV) tolerant oat germplasm lines. Our objective was to combine genes for tolerance to BYDV into germplasm lines with a higher level of tolerance to BYDV than the parents. The four-way cross involved four BYDV tolerant parents: IL86-1156, IL86-5698, IL86-6404 and Ogle. The F3 population was space-planted in the field in 1993 and inoculated with BYDV-PAV-IL when the plants were at Feekes GS 1. Plants that exhibited BYDV symptoms were destroyed. About 780 of the most tolerant plants (based on lack of symptoms) were harvested individually. In 1994, a single hill of each line was evaluated for BYDV tolerance in a BYDV-PAV-IL inoculated nursery, and 139 lines were selected. The 139 lines (plus the parents and checks) were evaluated in 1995 and 1996 using three replications of paired control and BYDV-PAV inoculated hills. Because these lines exhibit little or no BYDV symptoms, we evaluated BYDV tolerance based on virus titer using ELISA, percent stunting (height difference between control and inoculated hills), and percent yield loss (grain yield difference between control and inoculated hills). In 1996, 62 lines were evaluated for agronomic performance in a replicated experiment using 6 row plots at one location. In addition to BYDV tolerance, lines were selected for further evaluation based on grain yield per se, kernel morphology and absence of awns. In 1997, 42 lines were evaluated further in replicated trials at two locations. Based on all of the criteria used for selection, 7 lines are being increased for release as BYDV tolerant germplasm lines. These lines differ somewhat in height, maturity and virus titer, but all have excellent tolerance to BYDV-PAV.