Author
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Plattner, Ronald |
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Submitted to: Association Official Analytical Chemists Midwest Section Program Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 6/10/1998 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: After extraction and sample cleanup, many methods for analysis of mycotoxins commonly use HPLC to separate the target compounds from the remaining sample matrix. UV or fluorescent detectors are commonly used to achieve sensitive quantitation of the small amounts of analyte. These methods frequently require a derivatization step and sometimes cannot detect all the desired mycotoxin homologs. An alternative method is the direct coupling of the HPLC with a mass spectrometer using either APCI or Electrospray Ionization for detection. We routinely use LC/MS for the underivatized analysis of fumonisins, trichothecenes, and other mycotoxins. LC/MS can offer advantages over other detection methods. For example, in the case of fumonisins, the fluorescent method can only detect the B series while LC/MS measures the A, B and P series. In the case of trichothecenes, type B trichothecenes have a convenient UV chromophore while type A do not. Sensitivity of detection for underivatized mycotoxins using MS detection is in the low nanogram range, making the overall sensitivity of the analytical method comparable with that obtained for UV and even fluorescent derivatives. |