Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/1998
Publication Date: N/A
Technical Abstract: In our ongoing research on the impact of genetic modification of oilseeds on food oil quality and utilization of vegetable oils, we studied the minor constituent distribution of canola oils. Two series of canola oils derived from different lines of genetically modified canola seeds along with control standards were analyzed for phospholipids, tocopherols, and phytosterols by various chromatographic techniques. Phospholipids were enriched by silica gel chromatography. As in modified soybean oils, there was a decrease in content and composition of phosphatidic acid in three of the modified crude oils derived from one of the two canola seed lines investigated. Normal phase high performance liquid chromatographic (HPLC) analysis of phospholipid isolates showed definite but small variations in the content of other major phospholipid classes despite few differences in their composition. Analysis of reversed phase HPLC data indicated that the edistribution of phosphatidylethanolamine molecular species was more significantly affected by genetic modification than phosphatidylcholine species. Impact of oilseed modification on the tocopherol content was rather erratic, although greater variability was manifested in the concentration of alpha and gamma tocopherols than delta tocopherol in modified canola oils. Phytosterol levels were dramatically influenced by modification of oilseeds. Thus, contents of the three phytosterols detected were lowered consistently in one series of modified oils, whereas discernible increases in the brassicasterol content of another series of canola oil varieties were observed. In general, genetic modification of canola seeds led to variable changes in the distribution of minor constituents of the oils.