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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #91470
Title: A GROUP I INTRON AS A MOLECULAR MARKER DIFFERENTIATING ADZUKIBEAN ISOLATES FROM SOYBEAN ISOLATES OF PHIALOPHORA GREGATA

Author
item CHEN, WEIDONG - UNIV OF ILLINOIS
item Gray, Lynn
item GRAU, CRAIG - UNIV OF ILLINOIS

Submitted to: North Central American Phytopathology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/10/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: An insertion sequence was detected near the 3' end of the nuclear 18S rDNA in isolates of P. g. f. sp. adzukicola causing brown stem rot (BSR) of adzuki bean. This insertion sequence was absent in isolates of P. g. f. sp sojae which causes BSR of soybean. The insertion sequence is 304 bp long and contains all the features of group I introns. These features include the four conserved sequence elements (P, Q, R, and S), the presence of a U at the 5' splice site of the exon, a G at the 3' splice site of the intron, a putative internal guiding sequence, and the sequence fit a secondary structure model for group I introns. Like most group I introns in nuclear 18S rDNA, this intron is located at a highly conserved region and is devoid of long open reading frames. This intron provides a convenient marker for use in conventional PCR to differentiate P. g. f. sp. adzukicola from P. g. f. sp. sojae.