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ARS Home » Research » Publications at this Location » Publication #91277
Title: COMPARISON OF LIQUID MODIFIERS USED WITH SUPERCRITICAL CARBON DIOXIDE AND THEIR EFFECT ON ANALYTE YIELD AND THE AMOUNT OF INTERFERENCES IN THE RESULTANT EXTRACT

Author
item JARVENPAA, EILA - UNIV OF TURKU, FINLAND
item HUOPALAHTI, RAINER - UNIV OF TURKU, FINLAND
item King, Jerry
item Taylor, Scott

Submitted to: Supercritical Fluids International Symposium
Publication Type: Proceedings
Publication Acceptance Date: 3/24/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Rolled oat samples spiked with deoxynivalenol (DON) were extracted using an ISCO Model 3560 automated supercritical fluid extractor, equipped with dual pumping capability. Methanol, acetontrile, water and their mixtures were used as static and dynamic modifiers with supercritical carbon dioxide. Initially, the extraction results using just methanol were compared with those using acetonitrile, and mixtures of these two liquids as a modifier. The identity and concentration of the modifier had a prominent effect on the amount of interferences found in the HPLC analyses as judged from the total HPLC chromatogram peak area. The highest recovery of DON was obtained with methanol, but mixtures of methanol and acetonitrile gave similar results. Next, liquid modifiers were added directly onto the sample prior to SFE, and the SFE was performed dynamically with acetonitrile as a modifier in carbon dioxide. The yield of hexane soluble fat increased as the amount of static modifier increased, the modifier being acetonitrile, or a mixture of methanol or acetontrile. In contrast, the amount of added acetonitrile/water (84:16) had no effect on the yield of fat. As more water was added as a static modifier, the less fat was extracted. However, the yield of the analyte was found to be higher when adding the 84:16 mixture of acetonitrile/water, or neat water as static modifiers onto the sample. As a practical compromise, the 84:16 mixture was used as a dynamic modifier. The modifer concentration (5-20%) in the carbon dioxide affected the analyte yield, which ranged from 40 to over 100% recovery. However, the effect on the fat yield was negligible.