Submitted to: Analytical Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/8/1998
Publication Date: N/A
Technical Abstract: The quantitative detection of oocysts of the protozoan parasite Cryptosporidium in water, biofilms, soils, sediments, clinical specimens, veterinary specimens, aerosols, waste treatment, water treatment filters, backwash of filters, purification columns and membranes, or biofilms on surfaces, is based on signature lipid biomarker analysis based on the detection of 10 hydroxy steraric free acid. The molecular structure is determined after quantitative recovery of the oocysts, extraction of the lipids, fractionation of the lipids on a silicic acid column, separation of the free fatty acid fraction by high performance, liquid chromatography, and detection as negative ions by mass spectrometry after electrospray ionization. The procedure has the sensitivity for detection of less than a single oocyst.