Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/24/1998
Publication Date: N/A
Technical Abstract: During the last three years we have utilized recombinant DNA, polymerase chain reaction (PCR) and nucleotide sequence technologies to investigate possible natural infection of wild and cultivated pears (Pyrus amygdaliformis and P. communis, respectively) in Greece with peach latent mosaic viroid (PLMVd). Natural infection of Greek pears with apple scar skin viroid (ASSVd) and pear blister canker viroid (PBCVd) was also investigated. Hybridization experiments of pear total nucleic acids from leaf, fruit or bark tissue of Greek pear and wild pear with DIG-11-labeled PLMVd, ASSVd, or PBCVd cRNA probe revealed PLMVd, ASSVd and PBCVd related sequences in wild and cultivated pears. When PLMVd primers were used for RT-PCR, an amplified DNA of the expected size (ca. 337 bp) was obtained. Similarly, when ASSVd primers were used for RT-PCR, amplified DNA of the expected size (330 bp) was also detected. Products of the expected size were also obtained in RT-PCR assays, using PLMVd primers, with nucleic acid extracts of an Italian pear cultivar from Bologna. Cloning and sequencing of DNA that was RT-PCR amplified with PLMVd primers, revealed typical PLMVd nucleotide sequence from naturally infected Greek and Italian pears with a chain length of 338 nucleotides.