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ARS Home » Research » Publications at this Location » Publication #89939
Title: MOLECULAR GENETIC ANALYSIS OF BLAST RESISTANCE IN CO39 CORRESPONDING TO AVIRULENCE GENE AVR1-CO39 OF MAGNAPORTHE GRISEA

Author
item CHAUHAN, R - UNIVERSITY OF WISCONSIN
item FARMAN, MARK - UNIVERSITY OF KENTUCKY
item RONALD, P - UNIVERSITY OF CALIFORNIA
item Leong, Sally

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/4/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Currently we are using different strategies for cloning the blast resistance gene in CO39. Fifteen mirosat (simple sequence repeats) markers, mapping to four rice chromosomes 1, 6, 11 & 12, known to carry disease resistance genes, were tested for polymorphism between CO39 (resistant) and 51583 (susceptible). Most of the test RM markers showed polymorphism between the resistant and susceptible lines. Co-segregation of polymorphic RM markers with resistance/susceptibility was tested using bulked segregant analysis as well as individual F3 families. Microsat marker, RM202, located on chromosome 11, co-segregated with blast resistance in resistant/susceptible F3 families as well as bulks. Linkage relationship between RM202 and resistance gene in CO39 is being established by testing the response of segregating F2 populations. RFLP markers bracketing RM202 on chromosome 11 are also being tested for cosegregation with the target resistance gene. Molecular markers linked to the resistance gene will be used to identify BAC clones of CO39 for eventual cloning of target gene using "Gene Golfing." Structural homologies between R genes from different plant species have facilitated the cloning of R gene from monocots using degenerate oligos from dicot R genes (2). This strategy is also being used for cloning the R gene in CO39. Degenerate oligonuceotide primers designed by P. Ronald from the conserved regions of both the leucine-rich repeats (LRR) and serine-threonine kinase domains of Xa21, have amplified a 850bp fragment in CO39 and 51583.