|Leathers, Timothy - Tim|
Submitted to: Biosensors and Bioelectronics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 4/24/1998
Publication Date: N/A
Citation: Interpretive Summary: Valuable sugars can be recovered from low-value agricultural residues such as corn fiber and fermented to produce a variety of value-added products such as xylitol. Practical new technologies are needed to detect and monitor these sugars and their fermentation products in real-time under field and factory conditions. Currently available methods suffer from slow response times, high maintenance and high cost. Biosensors (electronic instruments utilizing bacterial cells) are rugged, inexpensive, reliable and simple to operate. However, biosensors are limited in their ability to detect low levels of sugars. This work will be of interest to those developing new uses and value- added products from agricultural commodities and byproducts and will in turn benefit farmers by fostering new and expanded markets for their products.
Technical Abstract: Amperometric sensors containing immobilized cells of Gluconobacter oxydans were hyperoxygenated to 400% of control levels and the effects determined on sensor responses to glucose. Oxygenated perfluorodecaline (a completely fluorinated organic substance) was as effective in hyperoxygenation as direct sparging with O2, increasing sensor base medium oxygen concentrations from 9.3 to 37 mg/l. Hyperoxygenation enhanced maximal biosensor response amplitudes, particularly at high cell loading densities. Maximal response rates were also improved, although less dramatically. Results suggest that hyperoxygenation may be a new general approach for modulating biosensor responses.