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Submitted to: American Dairy Science Association Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 4/20/1998 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Staphylococcus aureus is a major cause of the $2 billion loss due to mastitis in dairy cattle. Staphylococcus aureus have the ability to adhere and penetrate mammary tissue and form deep seated abscesses which cause chronic cases of mastitis that necessitate culling the cow. Microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) on the surface of the S. aureus mediate the adherence to mammary tissue. S.aureus strain M60 was evaluated for its ability to adhere to collagen or fibronectin coated dishes. Recombinant collagen and fibronectin binding MSCRAMMs, an anti-MSCRAMM antibody and mutant strains of S. aureus that expressed or failed to express the fibronectin and/or collagen binding MSCRAMMs were also used to evaluate adherence of S. aureus to bovine mammary epithelial cell monolayers. M60 bound significantly less to collagen than to fibronectin (P<0.05). Collagen binding MSCRAMMs had no significant effect on adherence (P<0.05). Fibronectin binding MSCRAMMs blocked adherence of S. aureus P<0.01). Anti-MSCRAMM antibody blocked adherence (P<0.05). Deletion of the genes for the fibronectin binding MSCRAMM significantly reduced adherence of S. aureus (P<0.05). Delection of the gene for the collagen binding MSCRAMM had no significant effect (P<0.05). The fibronectin binding MSCRAMM appeared to play an important role in adherence of S. aureus. These data suggest that recombinent fibronectin binding MSCRAMs and rabbit anti-MSCRAMM antibody have promise as a means of preventing S. aureus adherence. Thus immunization of cows with fibronectin binding MSCRAMMs could aid in the prevention of S. aureus mastitis in cattle. |
