MOLECULAR MARKERS LINKED TO QUANTITATIVE TRAIT LOCI FOR ANTHRACNOSE RESISTANCE IN TOMATO

Authors: Stommel, John; Zhang, Yiping

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Random amplified polymorphic DNA (RAPD) and amplified fragment
length polymorphism (AFLP) markers linked to quantitative trait
loci (QTL) involved in tomato anthracnose resistance were
identified in an F2 population of tomato (Lycopersicon
esculentum) segregating for anthracnose resistance. The F2
population was developed from the cross of an unadapted and
small-fruited, but highly anthracnose resistant L. esculentum
accession and an adapted, but anthracnose susceptible processing
type tomato. Resistance to anthracnose caused by the fungal
pathogen Colletotrichum coccodes is estimated to be controlled by
at least three genes or chromosomal regions in this cross.
One-thousand RAPD random primers and 64 AFLP primer pairs were
screened for polymorphisms between the parental lines. Primers
or primer pairs which differentiated the anthracnose resistant
and susceptible parents were utilized to screen the F2 population
for detection of QTL. Using single-factor analysis of variance,
a number of markers, including six unmapped RAPD markers were
identified which were significantly associated with resistance.
Mapping of marker loci and their potential use in marker assisted
breeding will be discussed.