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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #86855
Title: FLOW CYTOMETRIC ANALYSIS OF PHIALOPHORA GREGATA

Author
item YEATER, KATHLEEN - UNIV OF ILLINOIS
item RAYBURN, ALBERT - UNIV OF ILLINOIS
item Gray, Lynn

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Recently, flow cytometry has been used to characterize conidia of Phialophora gregata isolated from soybean vascular tissue. The conidia are stained with a DNA fluorochrome and are analyzed based on their fluorescence intensity. In order to take full advantage of the flow cytometric technique, the choice of fluorochrome is critical. Most studies to date have been restricted to propidium iodide, ethidium bromide, mithramycin, and 4'6-diamidino-2-phenyl-indol-dihydorochloride. The purpose of this study was to screen recently developed DNA fluorochromes for their ability to stain fungal conidia. The fluorochromes tested were YOYO-1, TOTO-1, and POPO-1. The conidia were stained and run through an EPICS 751 (Coulter Corporation) flow cytometer cell sorter with CICERO software. Upon comparison of staining technique, flow cytometric technique, and flow cytometric analyses tested, YOYO-1 was found to be the fluorochrome of choice in fungal spore analyses.