Skip to main content
ARS Home » Midwest Area » Columbia, Missouri » Biological Control of Insects Research » Research » Publications at this Location » Publication #86050

Title: PRELIMINARY IDENTIFICATION OF PROTEINS IN SALIVA OF THE POTATO LEAFHOPPER

Author
item HABIBI, JAVAD
item BACKUS, ELAINE
item Coudron, Thomas
item BRANDT, SANDRA

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: To elucidate the molecular mechanism of hopperburn, we sought to determine the major components of potato leafhopper saliva. To obtain saliva proteins, about 200 adult potato leafhoppers were fed on diet cubes comprised of agarose and sucrose. Proteins were electro-eluted from the diet cubes into a buffer. To obtain salivary gland proteins, glands were dissected from adult potato leafhopper, and the soluble protein content wa actively diffused into a buffer. The protein content from both saliva and salivary glands were separated by charge and size using vertical and continuous flow electrophoresis.