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ARS Home » Research » Publications at this Location » Publication #85447
Title: VIRULENCE AND MOLECULAR POLYMORPHISM OF PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES

Author
item Hammond, Rosemarie
item Crosslin, James

Submitted to: Journal of General Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/9/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Prunus necrotic ringspot ilarvirus (PNRSV) is a serious pathogen of many woody plant species including peach, cherry, rose, apple, and plum. Sweet cherry trees infected with PNRSV exhibit a range of symptoms from none to severe rugose mosaic disease, with resulting losses in fruit yield and marketability, and tree decline. In this study, we report sequence comparison of seven biologically well-characterized isolates of PNRSV and the resulting correlation between sequence and symptom type. The results will be of value to scientists in providing a basis for designing rapid type-specific diagnostic assays for field applications.

Technical Abstract: Prunus necrotic ringspot virus (PNRSV) occurs as numerous strains or isolates that vary widely in their pathogenic, biophysical, and serological properties. The nucleic acid sequence was determined from 1.65kbp RT-PCR products derived from RNA 3 of 7 distinct isolates of PNRSV that differ serologically and in pathology on sweet cherry. Sequence comparisons of ORF 3a (putative movement protein) and ORF 3b (coat protein) revealed single nucleotide and amino acid differences with strong correlations to serology and symptom types (pathotypes). Sequence differences between serotypes and pathotypes were also reflected in the overall phylogenetic relationships between the isolates.