Submitted to: Journal of the American Oil Chemists' Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/23/1998
Publication Date: N/A
Interpretive Summary: The United States has an annual carry-over of 1.2 billion pounds of soybean oil more than what is consumed. It would be economically beneficial to find new non-food uses for this commodity. A microorganism has been found which adds reactive sites to a component of soybean oil. This changes the properties of this component and enhances the potential use in industries that produce coatings, detergents, polymers and lubricants.
Technical Abstract: Fifty-eight Bacillus species originally isolated from various intestines were obtained from the ARS Culture Collection to investigate their ability to bioconvert oleic acid to unique hydroxy or keto derivatives. Addition of 0.5% oleic acid substrate to a whole-cell culture of one Bacillus sp. (NRRC BD-447) produced mixtures of mono-, di-, and tri-substituted compounds. These products appear to contain combinations of keto- and hydroxy- groups. One of the major products was isolated and its structure determined by GC-MS and NMR. This compound is tentatively identified as 7-hydroxy-16-keto-9-octa- decenoic acid.