Submitted to: Journal of Genetics and Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/27/1996
Publication Date: N/A
Interpretive Summary: Chlorina or light-yellow mutants are very common in barley. They occur spontaneously or may be induced by chemicals. Many of them are morphologically similar. However, they may be located in different chromosomes or in different positions on the same chromosome. The same chlorina gene may also express slightly different in different plants depending on their genetic background. An allelism test is designed to se if similar chlorina mutants are controlled by the same or different genes. For the test, two chlorina mutants are crossed together. If the progenies from the first generation or F1 and from the second generation or F2 (derived from self pollination of the F1) are all chlorina, they are then controlled by the same gene. If the progenies in the F2 segregate, they are controlled by different genes. Another method which is used is maternal inheritance test. A chlorina is used as a female and crossed to a adominant green plant. If all the F1 plants are chlorina, the mutant shows maternal inheritance. If the chlorina is used as a male in the cross, the F1 plants are all green. In this report, twelve new chlorina mutants were tested for maternal inheritance; none of them showed this character. Eleven pairs were tested for allelism; six pairs were allelic to each other or controlled by the same gene. These tests are usually performed before mapping a gene on the chromosome. Lack of the allelism test may result in assigning different symbols for the same gene.
Technical Abstract: Various chlorina mutants from barley genetic stocks were tested for maternal inheritance. Allelic relations between new mutants and established f series chlorina stocks and allelism among those of similar phenotypes were also tested. Twelve stocks of chlorina mutants did not show maternal inheritance. Among seven chlorina mutants tested for allelic crelations with established chlorina stocks, three of them showed allelic relations with fc or f genes and four of them did not show allelic relations with f3, f5, or f7 genes. In addition, four pairs of chlorina stocks showed similar phenotype with each other, while three groups showed allelism and one group did not. The results of maternal and allelism studies and their significance in genetic studies are discussed.