Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/1997
Publication Date: N/A
Technical Abstract: Fully degenerate polymerase chain reaction primers, designed to represent amino-acid conserved regions between putative transposase genes of the Drosophila mauritiana and Hyalophora cercopia mariner transposable elements, were used to screen genomic DNA of 18 established insect cell lines for possible mariner-like elements. All cell lines except Aedes albopictus were positive yielding a single appropriate size band of approximately 490 bp. The PCR product in the 490 bp region from one of these insect cell lines, S. frugiperda (Sf21), was further characterized by cloning and sequencing to confirm whether it was a mariner-like transposon element. Alignment of the nucleotide sequences of three clones of the S. frugiperda PCR fragment showed that rather than being similar to the mariner transposon gene of D. mauritiana and H. cercopia, sequences were aligned to the Anopheles albimanus clone Que 13 transposon transposase gene, a member of the Tc1 family of transposons. Further cloning and sequencing of the S. frugiperda cell line will be conducted to confirm these initial findings as well as to investigate several other insect cell lines for the presence of the Tc1 transposon.