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ARS Home » Midwest Area » Wooster, Ohio » Application Technology Research » Research » Publications at this Location » Publication #81742
Title: ISOLATION AND INFECTIVITY OF ENTOMOPATHOGENIC NEMATODES FROM OHIO

Author
item Klein, Michael
item Moyseenko, James

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 8/8/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Although golf courses are treated with many pesticides, epizootics of Heterorhabditis megadis, H. bacteriophora, and Steinernema carpocapsae have been noted in larvae of the Japanese beetle, Popillia japonica. We surveyed golf course turf in northern Ohio over a five year period, and isolated nematodes from about 20% of the samples using the galleria bait technique. Roughly 70% of all isolates were Steinernema spp. All isolates of heterorhabditids were H. bacteriophora. Despite numerous attempts, we were unable to reisolate H. megadis from its original location on a Ashland County Ohio golf course. No isolates of the scarab pathogen S. glaseri were found. Only five of 32 isolates of Steinernema spp. tested in the laboratory gave more than 75% control of scarab larvae. Nineteen of 20 of the H. bacteriophora isolates caused that level of control. Selected isolates of our H. bacteriophora performed as well in field tests as did commercially available nematodes.