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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Publications at this Location » Publication #81640
Title: AN RFLP LINKAGE MAP OF UPLAND COTTON (GOSSYPIUM HIRSUTUM L.)

Author
item SHAPPLEY, ZACHARY
item Jenkins, Johnie
item Meredith Jr, William
item McCarty, Jack

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/29/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Molecular markers in upland cotton will be useful to breeders and geneticists. They will become more useful when associations between molecular markers and traits of commercial value are known. The first steps are to identify molecular markers and determine their linkage relationships. Using RFLP screening of an F2 cross of two upland cultivars, we have identified the location of 128 molecular marker loci and arranged them into 31 linkage groups covering 18% of the estimated length of the cotton genome. Each linkage group contained from 2 to 10 molecular loci and ranged from 0.5 to 107 cM in length. We also discovered 18 unlinked molecular loci. This accomplishment represents the most complete RFLP map available for a cross between two upland cotton cultivars. It also forms the foundation for determining associations between these molecular markers and traits of commercial value.

Technical Abstract: Ninety-six individual bulk-sampled plots of an F2-derived F3 population of upland cotton (Gossypium hirsutum L.) of the cross HS46 x MARCABUCAG8US-1-88 (MAR) were analyzed with 137 probe/enzyme combinations resulting in 146 loci from 259 polymorphic fragments. Eighty-nine of the loci were identified as codominant while 57 were identified as +/- genotypes. These 146 loci were analyzed with the MAPMAKER\EXP program to determine linkage relationships among them. One-hundred-and-twenty-eight loci were arranged into 31 linkage groups covering 897.7 cM, or an estimated 17.5% of the cotton genome. The linkage groups ranged from 2 to 10 loci each and ranged in size from 0.5 to 107 cM. Eighteen non-linked loci were found.