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ARS Home » Research » Publications at this Location » Publication #81317
Title: BOVINE NEUTROPHIL FUNCTIONS DURING DIAPEDESIS THROUGH THE BLOOD/MILK BARRIER

Author
item SMITS, E
item ROET, E
item Guidry, Albert
item CIFRIAN, E
item RAINARD, P
item BURVENICH, C

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Neutrophils play a crucial role in host defense against bacterial infection in the bovine mammary gland. In order to fulfill their function neutrophils must pass the endothelial cell layer, the interstitial matrix and the epithelial lining of the mammary gland. In vivo studies of this movement (diapedesis) are limited by the complexity of the mammary gland. The current study reports a cell culture system composed of two chambers separated by a confluent monolayer of epithelial cells and fibroblast supported by a permeable membrane. Chemotactic factors were added to the epithelial chamber to determine their effect on neutrophil movement through the monolayer. The complement component C5a, Escherichia coli endotoxin and E. coli-endotoxin activated serum were used as chemoattractants. C5a was the most potent chemoattractant. Escherichia coli endotoxin was not chemotactic. Serum alone was not chemotactic, but E. coli-endotoxin activated serum was almost as chemotactic as C5a. Also, when the activity of C5a in the serum was blocked with antibodies to C5a, there was significant chemotactic activity remaining in the serum. This indicated that there were factors other than C5a that were responsible for the chemotactic activity of E. coli-endotoxin activated serum. This study clearly demonstrated the value of an in vivo model for studying physiological phenomena involving the blood/milk barrier in mammary gland.