Submitted to: Methods in Molecular Biology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 3/1/1998
Publication Date: N/A
Citation: Interpretive Summary: Orchid cultivation is one of the most economically significant nursery industries in the United States constituting a multi-million dollar industry. Desirable and important plants can be reproduced using a process called tissue culture. Desirable orchids produced through laborious breeding processes can only be cloned through tissue culture. Hundreds of research papers dealing with orchid tissue culture have been published to date. Many of these papers conflict and often employ exotic and obscure formulations as well as unique cultural techniques. This paper was written to simplify and clarify the procedures involved in orchid tissue culture by presenting the necessary orchid tissue culture techniques and nutrients in an immediate usable format which can be employed for the propagation of diverse types of orchids. This paper essentially reduces orchid tissue culture down to two methodologies which will greatly reduce the effort, time, and expense necessary to reproduce orchids.
Technical Abstract: Methods for clonal propagation of the two major morphological groups of orchids, i.e., sympodials and monopodials, are presented. Sympodials are characterized by a multi-branching rhizome that can supply an abundance of axillary shoots for use as explants. Monopodials are characterized by a single, unbranched axis that possesses few available axillary shoots for use as explants. Sympodial clonal culture methods are further divided int two methods via protocorm-like bodies (PLB) or by axillary shooting. Monopodial clonal culture methods are achieved through PLB and axillary shooting methods also.