Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 10/31/1997
Publication Date: N/A
Technical Abstract: We used the single cell gel electrophoresis (Comet) assay and flow cytometry as a rapid and sensitive mammalian cell technique to analyze agricultural processing products for antimutagenic properties. The Comet assay detects DNA strand breaks after acute exposure to genotoxins while flow cytometry measures chromosome aberrations in Chinese hamster lung (CHL) YG10008 cells treated. We discovered that non-toxic concentrations of an ethanol extract (PCC) of commercial soybean processing by-product (soybean molasses) exhibited antimutagenic activity. In a PCC concentration range from 5 to 500 g/ml, a direct reduction in the Comet tail moment values was observed in CHL cells challenged with 500 nM 2-acetoxyacetylaminofluorene (2AAAF). At 500 g/ml, PCC eliminated the mutagenic activity of 2AAAF in the treated CHL cells. This reduction in genotoxic activity was also observed at the level of the chromosome using flow cytometry 48 h after treatment. When CHO AS52 cells were analyzed for induced forward mutation at gpt, PCC at a concentration of 500 ug/ml reduced the mutagenic activity of 500 nM 2AAAF by 90%. We fractionated PCC on a C-18 reverse phase column and generated a series of eluate fractions. The fraction that eluted with 100% methanol contained the majority of the antimutagenic activity. These data illustrate that commercial agronomic processing by-products contain antimutagenic agents that may have anticarcinogenic potential as food supplements. Research funded in part by the Dept. of Crop Sciences and C-FAR grant 95-70-4.