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United States Department of Agriculture

Agricultural Research Service

Title: EFFECTS OF RECOMBINANT BOVINE GRANULOCYTE-MACROPHAGE-COLONY STIMULATORY FACTOR ADMINISTRATION ON THE BOVINE IMMUNE SYSTEM AND RESISTANCE TO INTRAMAMMARY CHALLENGE WITH ESCHERICHIA COLI)

Author
item Kehrli Jr, Marcus
item Shuster, Dale
item Stabel, Judith
item Stevens, Mark
item Thurston, John
item Daley, Michael

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/1997
Publication Date: N/A
Citation:

Interpretive Summary:

Technical Abstract: An experiment was conducted to determine the immunomodulatory activity of recombinant bovine granulocyte-macrophage colony stimulatory factor (rbGM-CSF) in lactating dairy cows. Administration of rbGM-CSF was via single daily subcutaneous injection on alternating sides of the lateral cervical region at 0, 0.5, or 2.5 micrograms/kg body weight for eight consecutive days. Control cows received a placebo saline injection. Cows were challenged in one mammary quarter with 41 colony forming units of Escherichia coli after receiving seven daily injections of rbGM-CSF to measure any effects on experimentally-induced mastitis. A dose-dependent febrile response developed upon repeated administration of rbGM-CSF. Increases in rectal temperatures in response to rbGM-CSF administration after the first day averaged 0.37 degrees C greater than control cows within 5 to 6 h. By 9 to 10 h after rbGM-CSF injection, increases in rectal temperatures averaged 0.59 degrees C over control cows. Rectal temperatures returned to control levels by 24 h after each injection. Associated with the febrile responses were increases in respiration and heart rates.

Last Modified: 8/24/2016
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